ABSTRACT
Objective To explore changes in the vascular-protecting function of HDL in patients with diabetic retinopathy (DR).Methods Together 23 patients with diabetic retinopathy (DR,DR group) and 21 normal subjects (normal control group)were recruited,and the 2 mL blood were collected from each of these subjects for isolating high-density lipoprotein (HDL).And then human umbilical vein endothelial cells (HUVECs) treated with harvested HDL and PBS (blank control group) were used for detection of cell proliferation and migration in the three groups.Results Compared with the blank control group,HDL in the normal control can significantly promote the proliferation and migration of HUVECs;but when compared with the normal control group,the ability of HDL to promote cell proliferation and migration significantly decreased in the DR group,in which the proliferative capacity of the cells decreased by approximately 14.8％ (P =0.027 1),and the migration ability decreased by about 49.3％ (P =0.009 6).Conclusion HDL from DR patients is dysfunctional in stimulating endothelial cell migration and proliferation,which means that vascular-protecting function of HDL in patients with DR is abnormal.